FREIFELDER PHYSICAL BIOCHEMISTRY PDF

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DAVID FREIFELDER PHYSICAL BIOCHEMISTRY PDF

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Books by David Freifelder

As Dr Freifelder points out, there is a gap between the theoretical analyses of such techniques in textbooks of physical biochemistry, and the practical details in manuals usually relating to particular instruments. In this attempt to fill this gap, he avoids the mathematical derivations of formulae, concentrates on specifying the essential principles of each technique, indicates the sort of apparatus used, and gives examples of its application. The emphasis is on characterization of macromolecules, and the examples are taken predominantly from studies on nucleic acids, rather than the more usual applications to proteins. The book gives a comprehensive coverage of techniques including: direct observation by light and electron microscopy; general laboratory methods, with a good outline of radioactive counting; separation methods, particularly chromatography; hydrodynamic methods, emphasizing sedimentation techniques; and spectroscopic methods, which include absorbance, fluorescence, optical rotation and nuclear magnetic resonance. A discussion of X-ray crystallography is omitted on the reasonable grounds that, although it has made major contributions to the subject, it is too specialized for inclusion in the techniques selected. Within its terms of reference, this is an admirable book that should meet its specified aim of giving students an understanding of technical applications, and make the research literature somewhat more intelligible to the uninitiated.

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