Kiganris Crystal structure of the mismatch-specific thymine glycosylase domain of human methyl-CpG-binding protein MBD4. Post-replicative methylation of cytosine at the 5-position 5mC in DNA provides molecular basis of the epigenetic regulation of gene expression 1. These results together with previously published data suggest that in vivo both TDG and MBD4 play a role in the removal of deaminated 5hmC residues. Our work describes the first crystal structures of the catalytic domain of MBD4 in complex with mismatched bases located at the centre of a mer DNA duplex. Data collection and processing statistics are given in Table 1. Nevertheless, our biochemical data provide evidence for the role of MBD4 as an efficient back-up enzyme which can specifically act in densely methylated CpG regions of chromosomal DNA, where deamination of 5mC and 5hmC is expected to be more frequent.
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